The present study is aimed to evaluate the protective potential of curcumin on Aluminium chloride-induced neurodegeneration in D. melanogaster. Both genders of D. melanogaster (Harwich strain 1 to 3 days old) will be orally exposed to AlCl3 (10, 20, 50, 100 and 200 mg/kg diet) and curcumin (0.2, 0.5 and 1.0 mg/g diet) for 28 days survival and longevity assays respectively. Afterwards, the best dose will be selected based on the results from the survival and toxicity assays. Then, the rescue role of curcumin on Aluminium chloride-induced neurotoxicity in D. melanogaster after an established days of oral administration will be carried out using a wide spectrum of biochemical assays. Markers of neurotoxicity (negative geotaxis, acetyolinesterase (AChE), inflammation (nitric oxide (NO), oxidative stress-antioxidant status (hydrogen peroxide (H2O2), total thiol (T-SH), catalase and Glutathione-S-transferase), rate of emergence of offspring and histology of the brain will be evaluated.
The results from the experiments will help to unravel the possible rescue role of curcumin on AlCl3-induced neurotoxicity and aid in the design of concept for prevention and possible therapy for Alzheimer’s disease and occupational exposure to aluminum chloride.
